If we talk about whether the use of a frozen semen sample will affect the results, it will depend on what treatment is going to be carried out and what is the reason why it is decided to use a previously frozen sample and not a fresh sample.
It has been observed that freezing affects, to a greater or lesser extent, the mobility of the sperm, so in cases of severe male factor, the recommendation would be to use fresh semen.
In addition, donor semen samples are frozen (Spanish law does not allow the use of a fresh donor), so the use of frozen semen does not have to worsen the results except in certain particular cases.
On the other hand, the use of frozen samples allows us to have more flexibility when organizing treatments both for the needs of our patients and for the management of daily work in our clinics.